Talk:Amide Hydrogen Exchange

what is protection factor?

Resuspending lyophilized sample
http://dx.doi.org/10.1110/ps.073027007 used software "sphere", intensities on non-excangeable aliphatic protons as reference. instrument pre-tuned and pre-shimmed with an equivalent sample.

Dilution into D2O
http://dx.doi.org/10.1016/j.jmb.2007.10.033 2x dilution 10x dilution (also used CLEANEX-PM-FHSQC to measure H/H exchange)

Using desalting column
D2O sample into sephadex 25 spin column pre-equilibrated with H2O