Talk:Amide Hydrogen Exchange (HX)

What are the precision with which the exchange rate could be measured ? I read that it was already observed that a mutation, could cause slowering to up to 50 time, but I don't find the reference, if anyone find it, I'm interested in it.

Francois, motice "feedback feature" or "newsgroup" - it's not really a newsgrou but a comment system. It works though and is more responsive then wiki. I think precision of rate determination depends mostly on quality of data - S/N ratio in the decay curves. It can be really good or really bad. Evgeny Fadeev 12:48, 29 April 2008 (MDT)

Terminology
$$\displaystyle k_{ex}$$ EX2 mechanism, exclusion interaction, $$\Delta G_{ex}, m_{3,\Delta G}, k_{cl}, k_{op}, k_{rc}$$ salt

$$k_{rc}$$ - intrinsic exchange rate of amide proton

Pulse sequences
FHSQC, TROSY-HSQC salt

Other notes
electrostatic interaction for highly charged proteins salt


 * 1) salt pmid=17965190

Resuspending lyophilized sample
used software "sphere", intensities on non-excangeable aliphatic protons as reference. instrument pre-tuned and pre-shimmed with an equivalent sample.

Dilution into D2O
http://dx.doi.org/10.1016/j.jmb.2007.10.033 2x dilution 10x dilution (also used CLEANEX-PM-FHSQC to measure H/H exchange)

Using desalting column
D2O sample into sephadex 25 spin column pre-equilibrated with H2O