CBCACO (cbcacoA.c)

From NMR Wiki

This page is a part of the user-editable NMR Wiki pulse sequence database.

Disclaimer: You are solely responsible for correct use of these materials on your instrument.

search database

see edit instructions

see deposition instructions

report suggestions

Basic facts about cbcacoA.c

Overview

Pulse sequence correlates chemical shifts (within the same aminoacid) of Cβ,Cα in ω1 dimension with Cα in ω2 dimension and CO (carbonyl carbon) in ω3 dimension.

Detection is on ¹³C, so the pulse sequence is appropriate for paramagnetic proteins, where ¹H relaxation is too fast.

Selective 90 and 180 pulses are prepared by Pbox "on the fly".

Original publication[1] cites use of 13C-{1H,15N}TXO probe, protein was uniformly ¹³C, ¹⁵N and 70% ²H labeled.

References

1. Bermel, W and Bertini, I and Duma, L and Felli, IC and Emsley, L and Pierattelli, R and Vasos, PR. Complete Assignment of Heteronuclear Protein Resonances by Protonless NMR Spectroscopy. Angewandte Chemie , 2005. BibTeX [ross05]
2. http://www.cerm.unifi.it/home/research/cbcaco-ipap.html

   [link]