CON

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CON NMR experiment correlates amide 15N atoms of a residue (i) with 13C C' of residues (i) and (i-1) (a related experiment is CAN that instead correlates amide 15N with the Cα atoms in the same residue pattern).

CON is an 15N detected experiment, it also makes use of 13C, 1H and 2H spectrometer channels, so it needs a specialized probe that has those channels and has an inner coil optimized to detect 15N.

CON and CAN experiments were first developed 2010 in Prof. Wagner lab by Koh Takeuchi and coworkers.

Sample requirement: U-13C,15N,2H - uniform labeling in 15N, 13C and 2H. Concentrations ~ 1 mM.

CON experiment has the following advantages:

  • works for higher molecular weight proteins because it relies on slower relaxation of low gamma 15N nucleus.
  • provides high resolution in 15N dimension
  • works for proline-rich proteins as it does not rely on amide protons.

Implementations

References

  1. Takeuchi, K and Heffron, G and Sun, ZYJ and Frueh, DP and Wagner, G. Nitrogen-detected CAN and CON experiments as alternative experiments for main chain NMR resonance assignments. Journal of Biomolecular NMR pp. 1--12. BibTeX [cancon]
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