HCCH-COSY (G. Montelione, Rutgers Univ.)

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Basic facts about pfg_hcch_cosy_se4

Synopsis: HCCH-COSY straight-through experiment using C-C COSY transfer, based on ref.,modified for CT-C13 freq labeling and PFG with sensitivity enhancement


Sample requirements: U-13C or U-13C,15N

Code authors:

 C. Chien, G.T. Montelione

Copyright: G.T. Montelione, Structural Bioinformatics Laboratory, Center for Advanced Biotechnology and Medicine, Rutgers University

Console: Varian, Unity, 500 MHz

Hardware requirements:

 CO pulses are on RF channel 4

Download: readme, code, parameters, image

Package: CABM Pulse Sequence Library


  1. Kay, LE and Ikura, M and Bax, A. Proton-proton correlation via carbon-carbon couplings: a three-dimensional NMR approach for the assignment of aliphatic resonances in proteins labeled with carbon-13. Journal of the American Chemical Society 112(2):888--889, 1990. BibTeX [kay90]
  2. Bax, A and Clore, GM and Driscoll, PC and Gronenborn, AM and Ikura, M and Kay, LE. Practical aspects of proton-carbon-carbon-proton three-dimensional correlation spectroscopy of 13 C-labeled proteins. J Magn Reson 87:620--627, 1990. BibTeX [bax90]

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