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{{bruker-pulse-sequence
|name=selnogp
|synopsis=DPFGSE selective 1D NOESY
|family=[[DPFGSE 1D NOESY]]
|medium=Liquids
|author=
|dim=1
|nuclei=H
|observe=H
|comment=documented revision 1.11.2.3
}}
[[Category:NOESY]]
[[Category:DPGFSE]]

==Introduction==
The '''selnogp''' is a pulse sequence for Bruker epuipment. It is a selective 1D version of 2D NOESY using excitation sculpting technique with simple pulsed field gradients (PFG). this experiment can be used in place of traditional '''NOEDiff'''.

'''Standard parameters''' might be accessible on your system under name '''SELNOGP'''. If so, then typing '''rpar SELNOGP''' will load them, and only RF-pulse parameters will need to be adjusted.

This pulse sequence uses a single DPFGSE element for the selective excitation.

==Delays==
'''d1''' - recycling delay (1-5 x T1)

'''d8''' - mixing time (can be 500 ms for small molecules or 100 ms for larger molecules, such as peptides or proteins)

An [[NOE build up]] curve may be obtained by varying '''d8''' from small to larger values.

'''d16''' - gradient recovery delay (depends on the probe) may be on the order of 100-200 &mu;s.

==Carrier frequencies==
'''O1''' (or '''O1P''' for the value in ppm) is the carrier frequency used for the hard pulses.

Parameter '''spoffs2''' - may be used as an offset for the selective excitation.

If '''O1''' is set exactly at the selectively excited resonance, then '''spoffs2''' must be zero.

In most cases it will be more optimal to set '''O1''' at the center of spectrum and enter '''spoffs2''' as a difference in Hz between the resonance of interest and the carrier frequency applied to the hard pulses.

==Hard RF pulses==
'''p1''' - {{1H}} {{90}} pulse at power level '''pl1'''

Hard pulses are applied at O1 (or O1p) carrier frequency.

==Selective RF pulse==
There is one shaped selective pulse with the following adjustible parameters:

Note: '''pl0''' must be at '''120'''.

* '''p12''' - length (default 80 ms, but generally length should be chosen according to desired selectivity)
* '''sp2''' - power level
* '''spnam2''' - waveform file name (default '''Gaus1.1000''' - gaussian pulse with 1000 steps. Can be used a gaussian curve with 5% truncated to increase selectivity)
* '''spoffs2''' - offset in Hz relative to O1 - the carrier frequency

'''p12''' can be adjusted to the shortest value producing clean "parent" excited resonance signal at '''mixing time=0'''.

==Gradient pulses==

* '''p16''' (default 1 ms) - length used for the gradient pulses '''gp1''' and '''gp2'''
* '''gp1''' and '''gp2''' strengths of the two gradient pulses.
* '''gpnam1''' and '''gpnam2''' - shapes of gradient pulses (default - '''SINE.100''')

Suggested ratio of '''gp1'''/'''gp2''' - 15/40

==References==
;H. Kessler, H. Oschkinat, C. Griesinger & W. Bermel,
;   J. Magn. Reson. 70, 106 (1986)
;J. Stonehouse, P. Adell, J. Keeler & A.J. Shaka, J. Am. Chem. Soc 116,
;   6037 (1994)
;K. Stott, J. Stonehouse, J. Keeler, T.L. Hwang & A.J. Shaka,
;   J. Am. Chem. Soc 117, 4199-4200 (1995)

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Return to 1D NOESY for Bruker (selnogp).

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