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==Setup macro==
* dpfgse_satxfer - type that in vnmr or vnmrj and most parameters will be reasonably set
==RF parameters==
* '''pw''' ([[Pulse calibration|needs calibration]]), '''tpwr''' (typ. 62), '''tof''' - {{1H}} {{90}} pulse length, its power level and transmitter offset
* '''trim''', '''trimpwr''' - trim pulse length and power (applied at '''tof'''), on if '''trim_flg='y''''
* '''satpw''' (50 ms), '''satshape''' ('gauss'), '''satpwr''' (~[[RF pulse power|86 Hz peak power]]), '''satfrq''' (on intense protein peak, no ligand) - length, shape, power level, and transmitter offset for the selective protein saturation pulse.
* '''wrefpw''', '''wrefshape''', '''wrefpwr''', '''wrefpwrf''' (use only if wrefpw can't be adjusted, otherwise set to maximum) - length, shape, power level, [[fine power level]] for the solvent-selective {{180}} pulse used for the solvent suppression in the dpfgse part. Recommended wrefshape='square' - you should have file shapelib/square.RF on your system
* '''satfrqref''' (30 ppm) - transmitter offset far from any resonance

==Gradient parameters==
* '''gzlvl0''', '''gt0''' - part of gradient-{{90}}-gradient water suppression sequence activated by '''sspul='y''''
* '''gzlvl2''', '''gt2''' - gradient for dpfgse
* '''gstab''' - gradient recovery delay will depend on probe (typ. 100-200 ms)

==Delays==
* '''d1''' ('''>xferdly''') - relaxation delay - includes '''xferdelay'''. Actual relaxation delay is what is longer than '''xferdly'''
* '''xferdly''' (1.5-2s) - saturation transfer delay - part of '''d1'''.
* '''d3''' (50 ms) - delay between saturation pulses
* '''gstab''' - gradient recovery delay (see above)

==[[Varian flag variables|Flags]]==
* '''sspul''' - if '''='y'''' gradient-{{90}}-gradient scheme before '''d1''' will be in effect
* '''dpfgse_flg''' - if '''='y'''', [[dpfgse]] before acquisition will be used
* '''trim_flg''' - if '''='y'''', trim pulse will be used
* '''alt_grd''' - if '''='y'''', sign of the dpfse gradient will be alternated in '''+ + - -''' for each new scan.

[[Category:Varian]]
[[Category:Pulse sequences]]

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