NMR assignment of polynucleotides

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NOE-based correlations: imino protons → non-exchangeable aromatic protons → H1' ribose/deoxy-ribose[1].

Adenine and cytosine don't have imino groups.For these assignment of nucleobases can be done starting with across the base-pair NOEs. Article[1] discusses several experiments for the assignment of nucleobases.

References

  1. http://dx.doi.org/10.1007/s10858-009-9323-1 [schwalbe09]
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