CAN

From NMR Wiki

CAN NMR experiment correlates amide ¹⁵N atoms of a residue (i) with ¹³C Cα of residues (i) and (i-1) (a related experiment is CON that instead correlates amide ¹⁵N with the carbonyls in the same residue pattern).

CAN is an ¹⁵N detected experiment, it also makes use of ¹³C, ¹H and ²H spectrometer channels, so it needs a specialized probe that has those channels and has an inner coil optimized to detect ¹⁵N.

CAN and CON experiments were first developed 2010 in Prof. Wagner lab by Koh Takeuchi and coworkers.

Sample requirement: U-¹³C,¹⁵N,²H - uniform labeling in ¹⁵N, ¹³C and ²H. Concentrations ~ 1 mM.

CAN experiment has the following advantages:

• works for higher molecular weight proteins because it relies on slower relaxation of low gamma ¹⁵N nucleus.
• provides high resolution in ¹⁵N dimension
• works for proline-rich proteins as it does not rely on amide protons.

Implementations

• CAN for Bruker - pulse sequence to correlate CA and N (Wagner Lab)

References

1. Takeuchi, K and Heffron, G and Sun, ZYJ and Frueh, DP and Wagner, G. Nitrogen-detected CAN and CON experiments as alternative experiments for main chain NMR resonance assignments. Journal of Biomolecular NMR pp. 1--12. BibTeX [cancon]