Hen Lysozyme

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Early Work

Redfield, C., Dobson, C.M.. Sequential proton NMR assignments and secondary structure of hen egg white lysozyme in solution

Biochemistry 27(1):122--136, 1988

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A detailed example of purely ¹H assignment strategy. Thoughtful sample preparation to selectively show rapidly and slowly exchanging amide protons.

Protein Expression

@article{mackenzie1996epa,

 title=Template:Efficient production from Aspergillus niger of a heterologous protein and an individual protein domain, heavy isotope-labelled, for structure-function analysis,
 author={MacKenzie, D.A. and Spencer, J.A. and Gal-Co{\"e}ffet, M.F.L. and Archer, D.B.},
 journal={Journal of Biotechnology},
 volume={46},
 number={2},
 pages={85--93},
 year={1996},
 publisher={Elsevier}

}

Structure determination

Schwalbe, H., Grimshaw, S.B., Spencer, A., Buck, M., Boyd, J., Dobson, C.M., Redfield, C., Smith, L.J.. A refined solution structure of hen lysozyme determined using residual dipolar coupling data

Protein Science 10(4):677, 2001

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Used Aspergillus niger expression system by MacKenzie et al.