Hen Lysozyme

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Contents 1 Early Work 2 Protein Expression 3 Structure determination 4 Variable Pressure NMR

Early Work

Redfield, C., Dobson, C.M.. Sequential proton NMR assignments and secondary structure of hen egg white lysozyme in solution

Biochemistry 27(1):122--136, 1988

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A detailed example of purely ¹H assignment strategy. Thoughtful sample preparation to selectively show rapidly and slowly exchanging amide protons.

Protein Expression

MacKenzie, D.A., Spencer, J.A., Gal-Co{\"e}ffet, M.F.L., Archer, D.B.. Efficient production from Aspergillus niger of a heterologous protein and an individual protein domain, heavy isotope-labelled, for structure-function analysis

Journal of Biotechnology 46(2):85--93, 1996

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Structure determination

Schwalbe, H., Grimshaw, S.B., Spencer, A., Buck, M., Boyd, J., Dobson, C.M., Redfield, C., Smith, L.J.. A refined solution structure of hen lysozyme determined using residual dipolar coupling data

Protein Science 10(4):677, 2001

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Used Aspergillus niger expression system by MacKenzie et al.

Variable Pressure NMR

anonymous. {esponse of native and denatured hen lysozyme to high pressure studied by 15N/1H NMR spectroscopy},

 author={Kamatari, Y.O. and Yamada, H. and Akasaka, K. and Jones, J.A. and Dobson, C.M. and Smith, L.J.},
 journal={FEBS Journal},
 volume={268},
 number={6},
 pages={1782--1793},
 year={2001},
 publisher={FEBS}
 
BibTeX