2D JHMBC

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Varian and Bruker pulse program code available here

Related manuals

Analysis JHMBC using Sparky

Overview

H-X coupling measured as doublet splitting in F1 dimension divided by a preset integer scaling factor (~20). Values of J H-X as small as 0.9 Hz could be reliably determined.

First four pulses together with gradients suppress magnetization from 13C-attached protons, while retaining magnetization of protons bonded to magnetically-silent 12C nuclei.

Assigning couplings in this experiment is straightforward as chemical shifts in both dimensions are those of coupled nuclei.

Pulse sequence image

References

Meissner, A., Sorensen, O.W.. Measurement of J (H, H) and long range J (X, H) coupling constants in small molecules. Broadband XLOC and J-HMBC

Magn. Reson. Chem 39:49--52, 2001
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