Hydrogen bonding in proteins

From NMR Wiki

Baxter et al. [1] showed that temperature dependence of amide proton chemical shift is a good indicator of hydrogen bonding. A temperature coefficient above -4.5 ppb/K indicated presence of hydrogen bond in BPTI and egg hen lysozyme. This criterion had successfully delineated non-hydrogen bound protons that are in slow exchange with solvent due to being buried in the core of the protein.

References

1. Baxter, NJ and Williamson, MP. Temperature dependence of 1 H chemical shifts in proteins. Journal of Biomolecular NMR 9(4):359--369, 1997. BibTeX [baxter97]