2D JHMBC

From NMR Wiki

Varian and Bruker pulse program code available here

Related manuals

Analysis of JHMBC using Sparky

Implementations

• J-HMBC for Bruker (hmbcetgpjcl2nd)

Overview

H-X coupling measured as doublet splitting in F₁ dimension divided by a preset integer scaling factor (~20). Values of J H-X as small as 0.9 Hz could be reliably determined.

First four pulses together with gradients suppress magnetization from ¹³C-attached protons, while retaining magnetization of protons bonded to magnetically-silent ¹²C nuclei.

Assigning couplings in this experiment is straightforward as chemical shifts in both dimensions are those of coupled nuclei.

References

1. Meissner, A and Sorensen, OW. Measurement of J (H, H) and long range J (X, H) coupling constants in small molecules. Broadband XLOC and J-HMBC. Magn Reson Chem 39:49--52, 2001. BibTeX [meissner01]