NMR assignment of polynucleotides
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Article<cite>schwalbe</cite> discusses several experiments for the assignment of nucleobases, including HNN-COSY,<sup>2</sup>J-{{15N}}-HSQC, TROSY-relayed HCCH-COSY, {{13C}}-HMBC and proposed an experiment called 3D-({{15N}},{{13C}},{{1H}})-HNHC. | Article<cite>schwalbe</cite> discusses several experiments for the assignment of nucleobases, including HNN-COSY,<sup>2</sup>J-{{15N}}-HSQC, TROSY-relayed HCCH-COSY, {{13C}}-HMBC and proposed an experiment called 3D-({{15N}},{{13C}},{{1H}})-HNHC. | ||
| - | The latter experiment is a combination of {{13C}}-HSQC and <sup>2</sup>J-{{15N}}-HSQC. Article <cite>schwalbe</cite> also contains a scheme of [[J coupling]]s in the adenine base. | + | The latter experiment is a combination of {{13C}}-HSQC and <sup>2</sup>J-{{15N}}-HSQC. Article <cite>schwalbe</cite> also contains a scheme of some [[J coupling]]s in the adenine base. |
==References== | ==References== | ||
Current revision
Assignment of nucleobase resonances
Conventional method uses NOE-based correlations: imino protons → non-exchangeable aromatic protons → H1' ribose/deoxy-ribose[1].
Adenine and cytosine don't have imino groups. For these, assignment of nucleobases can be done starting with the across the base-pair NOEs. Article[1] discusses several experiments for the assignment of nucleobases, including HNN-COSY,2J-15N-HSQC, TROSY-relayed HCCH-COSY, 13C-HMBC and proposed an experiment called 3D-(15N,13C,1H)-HNHC.
The latter experiment is a combination of 13C-HSQC and 2J-15N-HSQC. Article [1] also contains a scheme of some J couplings in the adenine base.
