Pre- heteronuclear biomolecular NMR works
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#red98 bibtex=@article{redfield1988spn, | #red98 bibtex=@article{redfield1988spn, | ||
title={Sequential proton NMR assignments and secondary structure of hen egg white lysozyme in solution}, | title={Sequential proton NMR assignments and secondary structure of hen egg white lysozyme in solution}, | ||
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url={http://dx.doi.org/10.1021/bi00401a020} | url={http://dx.doi.org/10.1021/bi00401a020} | ||
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[[Category:NMR Bibliography]] | [[Category:NMR Bibliography]] | ||
Revision as of 17:47, 21 October 2008
Article [1] presents nearly complete 1H assignment of 129 residue hen egg lysozyme based on Phase-sensitive 2D NOESY, Phase-sensitive COSY and RELAY and Double RELAY spectra culminating a 15 year effort of assigning lysozyme exclusively by 1H NMR. The article presents a detailed illustrated explanation of the sequential assignment process. Spectra were recorded at 35oC. Samples were 7 mM, pH=3.8. Total of four samples were analyzed:
- in 90% H2O/10% D2O,
- fully D2O exchanged sample repared by heating at 80oC for 10 min
- partially D2O exchanged sample prepared by dissolving protein in D2O at 35oC; this sample retained slowly exchanging amide protons
- reverse-exchanged sample prepared by dissolving lyophylized fully D2O exchanged sample in 80% H2O/20% D2O. Solid sample was first dissolved in D2O then a proportional amount of H2O was added; this sample was used to detect quickly exchanging amide protons
