Varian dpfgse satxfer.c saturation transfer with DPFGSE water suppression

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Setup macro

  • dpfgse_satxfer - type that in vnmr or vnmrj and most parameters will be reasonably set

RF parameters

  • pw (needs calibration), tpwr (typ. 62), tof - 1H 90o pulse length, its power level and transmitter offset
  • trim, trimpwr - trim pulse length and power (applied at tof), on if trim_flg='y'
  • satpw (50 ms), satshape ('gauss'), satpwr (~86 Hz peak power), satfrq (on intense protein peak, no ligand) - length, shape, power level, and transmitter offset for the selective protein saturation pulse.
  • wrefpw, wrefshape, wrefpwr, wrefpwrf (use only if wrefpw can't be adjusted, otherwise set to maximum) - length, shape, power level, fine power level for the solvent-selective 180o pulse used for the solvent suppression in the dpfgse part. Recommended wrefshape='square' - you should have file shapelib/square.RF on your system
  • satfrqref (30 ppm) - transmitter offset far from any resonance

Gradient parameters

  • gzlvl0, gt0 - part of gradient-90o-gradient water suppression sequence activated by sspul='y'
  • gzlvl2, gt2 - gradient for dpfgse
  • gstab - gradient recovery delay will depend on probe (typ. 100-200 ms)


  • d1 (>xferdly) - relaxation delay - includes xferdelay. Actual relaxation delay is what is longer than xferdly
  • xferdly (1.5-2s) - saturation transfer delay - part of d1.
  • d3 (50 ms) - delay between saturation pulses
  • gstab - gradient recovery delay (see above)


  • sspul - if ='y' gradient-90o-gradient scheme before d1 will be in effect
  • dpfgse_flg - if ='y', dpfgse before acquisition will be used
  • trim_flg - if ='y', trim pulse will be used
  • alt_grd - if ='y', sign of the dpfse gradient will be alternated in + + - - for each new scan.
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