Water flip-back

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Revision as of 21:47, 11 May 2009 (edit) Evgeny Fadeev (Talk | contribs) (New page: <biblio> #wfb bibtex=@article{grzesiek1993ins, title={{The importance of not saturating water in protein NMR. Application to sensitivity enhancement and NOE measurements}}, author={Grz...) ← Previous diff		Current revision (21:50, 11 May 2009) (edit) (undo) Evgeny Fadeev (Talk | contribs)
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		+	In water flip-back element a hard readout {{90}} pulse on {{1H}} is preceded by a selective {{90}} pulse on water resonance with phase opposite to that of the [[hard pulse]].
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		+	If {{1H}} magnetization was along z axis before the selective pulse, water resonance will stay along z and won't be observed.
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		+	==References==
	<biblio>		<biblio>
	#wfb bibtex=@article{grzesiek1993ins,		#wfb bibtex=@article{grzesiek1993ins,
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	}		}
	</biblio>		</biblio>
		+	[[Category:NMR concept]]
		+	[[Category:Water suppression]]

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Current revision

In water flip-back element a hard readout 90^o pulse on ¹H is preceded by a selective 90^o pulse on water resonance with phase opposite to that of the hard pulse.

If ¹H magnetization was along z axis before the selective pulse, water resonance will stay along z and won't be observed.

References

1. Grzesiek, S and Bax, A. The importance of not saturating water in protein NMR. Application to sensitivity enhancement and NOE measurements. Journal of the American Chemical Society 115(26):12593--12594, 1993. BibTeX [wfb]