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{{varian-pulse-sequence
|name=cbcacoA.c
|synopsis=3D {{13C}} observe CBCACO experiment
|family=[[CBCACO]]
|medium=liquids
|author=nm
|dim=3
|nuclei=CNHD
|image=[http://www.cerm.unifi.it/home/research/cbcaco-ipap.html]
|hardware_requirements={{13C}} detection probe with {{1H}}, {{2H}}, {{15N}} channels
|observe=C}}
===Overview===
Pulse sequence correlates chemical shifts (within the same aminoacid) of C&beta;,C&alpha; in &omega;1 dimension with C&alpha; in &omega;2 dimension and CO (carbonyl carbon) in &omega;3 dimension.

Detection is on {{13C}}, so the pulse sequence is appropriate for paramagnetic proteins, where {{1H}} relaxation is too fast.

Selective 90 and 180 pulses are prepared by Pbox "on the fly".

Original publication<cite>ross05</cite> cites use of 13C-{1H,15N}TXO probe, protein was uniformly {{13C}}, {{15N}} and 70% {{2H}} labeled.

===References===
<biblio>
#ross05 bibtex=@article{bermel2005cah,
  title={{Complete Assignment of Heteronuclear Protein Resonances by Protonless NMR Spectroscopy}},
  author={Bermel, W. and Bertini, I. and Duma, L. and Felli, I.C. and Emsley, L. and Pierattelli, R. and Vasos, P.R.},
  journal={Angewandte Chemie},
  year={2005},
  url={http://dx.doi.org/10.1002/ange.200461794}
}
#link http://www.cerm.unifi.it/home/research/cbcaco-ipap.html
</biblio>
[[Category:pbox]]
[[Category:paramagnetic]]

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