2D HSQC

From NMR Wiki

2D HSQC (Heteronuclear Single Quantum Coherence) experiment correlates chemical shifts of directly bound nuclei (i.e. two types of chemical nuclei). For example ¹H,¹⁵N-HSQC correlates chemical shifts within NH groups.

¹H,¹⁵N-HSQC and ¹H,¹³C-HSQC are frequently used for liquid-state protein NMR spectroscopy.

Chemical shift of one nucleus - usually ¹H is detected in the directly measured dimension, while chemical shift of second nucleus (heteronucleus or X-nucleus) is recorded in the indirect dimension.

Implementations

13C HSQC decoupler2 (Rutgers Univ.)

15N-HSQC, with 13C decoupling and SE 500 MHz Inova (G. Montelione, Rutgers Univ.)

13C-HSQC, 15N decoupling, SE, 500 MHz Inova (G. Montelione, Rutgers Univ.)