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1H,13C-HSQC of Sudan I dye
1H,13C-HSQC of Sudan I dye

2D HSQC (Heteronuclear Single Quantum Coherence) experiment correlates chemical shifts of directly bound nuclei (i.e. two types of chemical nuclei). For example 1H,15N-HSQC correlates chemical shifts within NH groups. For similar correlations over multiple bonds 2D HMBC experiment can be used.

1H,15N-HSQC and 1H,13C-HSQC are frequently used for liquid-state protein NMR spectroscopy.

Chemical shift of one nucleus - usually 1H is detected in the directly measured dimension, while chemical shift of second nucleus (heteronucleus or X-nucleus) is recorded in the indirect dimension.

Recently modified HMQC experiment allowed to measure correlation faster using Ernst-angle pulse instead of 90° pulse ( SOFAST HMQC )

HSQC is preferred over HETCOR due to its higer sensitivity.


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