2D HSQC
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2D [[HSQC]] (Heteronuclear Single Quantum Coherence) experiment correlates chemical shifts of directly bound nuclei (i.e. two types of chemical nuclei). For example {{1H}},{{15N}}-HSQC correlates chemical shifts within NH groups. | 2D [[HSQC]] (Heteronuclear Single Quantum Coherence) experiment correlates chemical shifts of directly bound nuclei (i.e. two types of chemical nuclei). For example {{1H}},{{15N}}-HSQC correlates chemical shifts within NH groups. | ||
Revision as of 04:06, 2 June 2010
2D HSQC (Heteronuclear Single Quantum Coherence) experiment correlates chemical shifts of directly bound nuclei (i.e. two types of chemical nuclei). For example 1H,15N-HSQC correlates chemical shifts within NH groups.
1H,15N-HSQC and 1H,13C-HSQC are frequently used for liquid-state protein NMR spectroscopy.
Chemical shift of one nucleus - usually 1H is detected in the directly measured dimension, while chemical shift of second nucleus (heteronucleus or X-nucleus) is recorded in the indirect dimension.
Recently modified HMQC experiment allowed to measure correlation faster using Ernst-angle pulse instead of 90° pulse ( SOFAST HMQC )
Implementations
- 13C HSQC decoupler2 (Rutgers Univ.)
- 13C-HSQC, 15N decoupling, SE, 500 MHz Inova (G. Montelione, Rutgers Univ.)
- 13C-HSQC, 600 MHz Inova (Rutgers Univ.)
- 15N-HSQC with T2 measurement (Rutgers Univ.)
- 15N-HSQC with T2 measurement, PFG version (Rutgers Univ.)
- 15N-HSQC, 600 MHz Inova (Rutgers Univ.)
- 15N-HSQC, with 13C decoupling and SE 500 MHz Inova (G. Montelione, Rutgers Univ.)
- BIP 13C-HSQC (xm gsHSQC Carbon.c)
- Ser/Thr-(HC)NH HSQC (J. Chugh and R.V. Hosur, TIFR)
- TROSY 500 MHz Inova (G.Montelione, Rutgers Univ.)
- TROSY 600 MHz Inova (Rutgers Univ.)
