2D HSQC

From NMR Wiki

2D HSQC (Heteronuclear Single Quantum Coherence) experiment correlates chemical shifts of directly bound nuclei (i.e. two types of chemical nuclei). For example ¹H,¹⁵N-HSQC correlates chemical shifts within NH groups.

¹H,¹⁵N-HSQC and ¹H,¹³C-HSQC are frequently used for liquid-state protein NMR spectroscopy.

Chemical shift of one nucleus - usually ¹H is detected in the directly measured dimension, while chemical shift of second nucleus (heteronucleus or X-nucleus) is recorded in the indirect dimension.

Recently modified HMQC experiment allowed to measure correlation faster using Ernst-angle pulse instead of 90° pulse ( SOFAST HMQC )

Implementations

• 13C HSQC decoupler2 (Rutgers Univ.)
• 13C-HSQC, 15N decoupling, SE, 500 MHz Inova (G. Montelione, Rutgers Univ.)
• 13C-HSQC, 600 MHz Inova (Rutgers Univ.)
• 15N-HSQC with T2 measurement (Rutgers Univ.)
• 15N-HSQC with T2 measurement, PFG version (Rutgers Univ.)
• 15N-HSQC, 600 MHz Inova (Rutgers Univ.)
• 15N-HSQC, with 13C decoupling and SE 500 MHz Inova (G. Montelione, Rutgers Univ.)
• BIP 13C-HSQC (xm gsHSQC Carbon.c)
• Ser/Thr-(HC)NH HSQC (J. Chugh and R.V. Hosur, TIFR)
• TROSY 500 MHz Inova (G.Montelione, Rutgers Univ.)
• TROSY 600 MHz Inova (Rutgers Univ.)