CT-13C CBCA(CO)NH and HBHA(CO)NH (G. Montelione, Rutgers Univ.)

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Name: pfg_C_cbcaconh_se20
Experiment family/families: CBCA(CO)NH, HBHA(CO)NH
Synopsis: (H)CBCA(CO)NH or HBHA(CO)NH straight-through triple resonance experiment using C-C COSY transfer, constant-time 13C version, with sensitivity enhancement option

Code authors:

 C.B. Rios, M. Tashiro, G.T. Montelione
Copyright: G.T. Montelione, Protein NMR Laboratory - Center for Advanced Biotechnology and Medicine, Rutgers University
Console: Varian, Inc. Unity, 500 MHz
Console requirements: four RF channels (applies to some CABM sequences)
Source: CABM Pulse Sequence Library
Download (external links):readme pulse sequence parameters (tar) figure (pdf)

References

  1. Grzesiek, S and Bax, A. Correlating backbone amide and side chain resonances in larger proteins by multiple relayed triple resonance NMR. Journal of the American Chemical Society 114(16):6291--6293, 1992. BibTeX [bax92]
  2. Grzesiek, S and Bax, A. Amino acid type determination in the sequential assignment procedure of uniformly 13 C/15 N-enriched proteins. Journal of Biomolecular NMR 3(2):185--204, 1993. BibTeX [bax93]

  3. MUHANDIRAM, DR and KAY, LE. Gradient-enhanced triple-resonance three-dimensional NMR experiments with improved sensitivity. Journal of magnetic resonance Series B(Print) 103(3):203--216, 1994. BibTeX [kay94]

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