CT-13C H(C)NH, (H)CNH, H(C)NH-TOCSY, C(C)NH-TOCSY (G. Montelione, Rutgers Univ.)

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Name: pfg_C_hacanh_se7
Synopsis: family of triple resonance experiments for H-CC-N-H straight-through, constant-time 13C version, with sensitivity enhancement

Code authors:

 C.B. Rios, M. Tashiro, W. Feng, G.T. Montelione
Copyright: G.T. Montelione, Protein NMR Laboratory - Center for Advanced Biotechnology and Medicine, Rutgers University
Console: Varian, Inc. Unity, 500 MHz
Console requirements: four RF channels (applies to some CABM sequences)
Source: CABM Pulse Sequence Library
Download (external links):readme pulse sequence parameters (tar) figure (pdf)

References

  1. LYONS, BA and MONTELIONE, GT. An HCCNH triple-resonance experiment using carbon-13 isotropic mixing for correlating backbone amide and side-chain aliphatic resonances in isotopically enriched proteins. Journal of magnetic resonance Series B(Print) 101(2):206--209, 1993. BibTeX [lyons93jmr]
  2. Lyons, BA and Tashiro, M and Cedergren, L and Bilsson, B and Montelione, GT. An improved strategy for determining resonance assignments for isotopically enriched proteins and its application to an engineered domain of staphylococcal protein A. Biochemistry 32(31):7839--7845, 1993. BibTeX [lyons93biochem]

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