CT-13C H(CCO)NH, (H)C(CO)NH, H(CCO)NH-TOCSY, C(CCO)NH-TOCSY (G. Montelione, Rutgers Univ.)
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{{rutgers-pulse-sequence | {{rutgers-pulse-sequence | ||
|name=pfg_C_hacaconh_se12 | |name=pfg_C_hacaconh_se12 | ||
- | |synopsis=family of HC(CO)NH straight-through triple resonance experiments; constant time {{13C}} evolution | + | |synopsis=family of HC(CO)NH straight-through triple resonance experiments; constant time {{13C}} evolution, with {{se}} option |
|author=cbr,mt,wf,gtm | |author=cbr,mt,wf,gtm | ||
|console=Unity | |console=Unity | ||
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|image=true | |image=true | ||
|nuclei=HCN | |nuclei=HCN | ||
+ | |sample={{UCN}} | ||
+ | |co=true | ||
|observe=H | |observe=H | ||
|dim=3 | |dim=3 |
Revision as of 20:18, 5 October 2008
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Name: pfg_C_hacaconh_se12 | |||||
Synopsis: family of HC(CO)NH straight-through triple resonance experiments; constant time 13C evolution, with sensitivity enhancement option | |||||
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Copyright: G.T. Montelione, Protein NMR Laboratory - Center for Advanced Biotechnology and Medicine, Rutgers University | |||||
Console: Varian, Inc. Unity, 500 MHz | |||||
Console requirements: four RF channels (applies to some CABM sequences) | |||||
Source: CABM Pulse Sequence Library | |||||
Download (external links):readme pulse sequence parameters (tar) figure (pdf) |
References
- Montelione, GT and Lyons, BA and Emerson, SD and Tashiro, M. An efficient triple resonance experiment using carbon-13 isotropic mixing for determining sequence-specific resonance assignments of isotopically-enriched proteins. Journal of the American Chemical Society 114(27):10974--10975, 1992. BibTeX
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Lyons, BA and Tashiro, M and Cedergren, L and Bilsson, B and Montelione, GT. An improved strategy for determining resonance assignments for isotopically enriched proteins and its application to an engineered domain of staphylococcal protein A. Biochemistry 32(31):7839--7845, 1993. BibTeX
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Tashiro, M and Rios, CB and Montelione, GT. Classification of amino acid spin systems using PFG HCC (CO) NH-TOCSY with constant-time aliphatic 13 C frequency labeling. Journal of Biomolecular NMR 6(2):211--216, 1995. BibTeX
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Feng, W and Rios, CB and Montelione, GT. Phase labeling of C- H and C- C spin-system topologies: Application in PFG-HACANH and PFG-HACA (CO) NH triple-resonance experiments for determining backbone resonance assignments in proteins. Journal of Biomolecular NMR 8(1):98--104, 1996. BibTeX
Categories: Unassigned experiment family | Sensitivity enhancement | Pulse sequences | CABM pulse sequences | Varian | Liquids | Unity | 500 MHz | 1H | 15N | 13C | Heteronuclear | 1H detected | 3D | Protein backbone | Protein sidechain | PFG | Constant time