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DEPT is an acronym for Distortionless Enhancement by Polarization Transfer.

This experiment allows to determine multiplicity of carbon atom substitution with hydrogens.

For this purpose, three experiments need to be recorded, where pulse with φ3 has flip angle of 45o (a), 90o (b), and 135o (c).

Signs of signals in these experiments will reveal the substitution of carbon atoms:

  • in a (DEPT-45) all signals will be positive
  • in b (DEPT-90) only signals of CH groups will show
  • in c (DEPT-135) signals from CH2 will be negative, while CH and CH3 - positive

Below is the pulse sequence diagram for DEPT.

DEPT pulse sequence - Distortionless Enhancement by Polarization Transfer



  1. Berger, S and Braun, S and Kalinowski, HO. 200 and more NMR experiments: a practical course. Wiley-Vch Weinheim, 2004. BibTeX [200+]
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