TPPI
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Spectral width SW is set as <math>SW=\frac{1}{\Delta T_1}</math>, where ΔT<sub>1</sub> - size of increment in the indirect timensions in seconds (and SW in Hz). | Spectral width SW is set as <math>SW=\frac{1}{\Delta T_1}</math>, where ΔT<sub>1</sub> - size of increment in the indirect timensions in seconds (and SW in Hz). | ||
===References=== | ===References=== | ||
| - | < | + | <biblio> |
| - | @article{marion1983aps, | + | #marion83 bibtex=@article{marion1983aps, |
title={Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of 1H-1H spin-spin coupling constants in proteins.}, | title={Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of 1H-1H spin-spin coupling constants in proteins.}, | ||
author={Marion, D. and Wuthrich, K.}, | author={Marion, D. and Wuthrich, K.}, | ||
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abstract={Two-dimensional correlated spectroscopy (COSY) is used for measurements of proton-proton spin-spin coupling constants in protein 1H NMR spectra. High digital resolution along the frequency axis ω2 is achieved by placing the carrier frequency in the center of the spectrum, using quadrature detection in both dimensions and presenting the spectrum in the phase sensitive mode. Compared to other techniques for studies of spin-spin coupling constants, COSY provides greatly improved spectral resolution. This is illustrated by experiments with H2O solutions of the small globular protein BUSI IIA (bull seminal inhibitor IIA).} | abstract={Two-dimensional correlated spectroscopy (COSY) is used for measurements of proton-proton spin-spin coupling constants in protein 1H NMR spectra. High digital resolution along the frequency axis ω2 is achieved by placing the carrier frequency in the center of the spectrum, using quadrature detection in both dimensions and presenting the spectrum in the phase sensitive mode. Compared to other techniques for studies of spin-spin coupling constants, COSY provides greatly improved spectral resolution. This is illustrated by experiments with H2O solutions of the small globular protein BUSI IIA (bull seminal inhibitor IIA).} | ||
} | } | ||
| - | </ | + | </biblio> |
| - | [[Category:Draft | + | [[Category:Draft artcles]] |
| + | [[Category:Data acquisiqion]] | ||
Revision as of 19:41, 28 January 2009
In TPPI or time-proportional phase incrementation method phase of preparation pulse is incremented by 90o for every increment of evolution delay T1.
Transmitter set at the center of spectral window.
Spectral width SW is set as 
, where ΔT1 - size of increment in the indirect timensions in seconds (and SW in Hz).
