NMR Experiments

From NMR Wiki

This page is for description of pulse sequences, special applications. Please add references to the original publications, some publications demonstrating use, known implementations on NMR platforms, and set-up instructions.

Protein NMR experiments (5)

Contents 1 Homonuclear 1.1 One dimensional 1.2 Two dimensional 2 Heteronuclear 2.1 Two dimensional 2.1.1 Isotope filtered experiments 2.2 Three-dimensional 2.2.1 Isotope filtered experiments 3 Specific to proteins 3.1 General protein experiments 3.2 Aminoacid-specific 4 Links

Homonuclear

One dimensional

1D NOESY

Two dimensional

J-coupling correlations: DQF-COSY (or DQ-COSY), MQ-COSY, TOCSY

NOESY

Heteronuclear

Two dimensional HX correlation: HSQC JHX coupling: JHMBC, HETLOC, pfg,se-HETLOC NOESY: Heteronuclear NOESY Isotope filtered experiments 13C-filtered NOESY using swept 13C inversion

Three-dimensional NOESY-HSQC TOCSY-HSQC HCCH-COSY 3D HCN and HNC NOESY Isotope filtered experiments X-filtered NOESY-HSQC X-filtered TOCSY-HSQC Isotope filtered 13C HMQC NOESY

Specific to proteins

Most of these require ¹³C and ¹⁵N isotope labeled samples. Many experiments in other categories can also be applied to proteins.

General protein experiments

HNCA

HNCO

CBCA(CO)NH

CBCANH

HBHA(CO)NH

Aminoacid-specific

HNN-ST (NMR Experiment)

Links

Carlsberg Laborartory Pulse Sequence Library

BMRB pulse sequence library

CABM NMR pulse sequence library (Rutgers Univ.)

Bax Lab pulse sequences