2D CC-CTUC Solids

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===Implementation===
===Implementation===
 +
Proton to Carbon CP followed by scalar coupling modulated homonuclear mixing with rSNOB soft pulses and independent [http://nmrwiki.org/wiki/index.php?title=SPINAL SPINAL] decoupling periods during mixing and acquisition.
 +
All needed files were catenated together, and so must be separated. The file CTUC_COSY.acq is the first block of text after the author contributions. CTUC_COSY.s begins with
All needed files were catenated together, and so must be separated. The file CTUC_COSY.acq is the first block of text after the author contributions. CTUC_COSY.s begins with
-
name "2D_CC_shell";
+
name "2D_CC_shell";
and ends with
and ends with
-
return;
+
return;
The next several files should be separated and placed in /export/homes/USERNAME/pulse_sequences/cpc/includes. Each new file begins with a long comment section.
The next several files should be separated and placed in /export/homes/USERNAME/pulse_sequences/cpc/includes. Each new file begins with a long comment section.

Revision as of 16:55, 5 September 2008

This page is a part of the user-editable NMR Wiki pulse sequence database.

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Name: hCC-2D-CTUC COSY
Experiment family/families: solids
Synopsis: Constant-time Through-bond Carbon 2D correlation experiments
Implementation author(s): Lindsay Sperling, Gemma Commellas, Trent Franks, Chad Rienstra
Copyright: not assigned
Console: Varian, Infinity Plus, 500 MHz
Download: code, no sample/parameter data yet

Implementation

Proton to Carbon CP followed by scalar coupling modulated homonuclear mixing with rSNOB soft pulses and independent SPINAL decoupling periods during mixing and acquisition.

All needed files were catenated together, and so must be separated. The file CTUC_COSY.acq is the first block of text after the author contributions. CTUC_COSY.s begins with

name	"2D_CC_shell";

and ends with

return;

The next several files should be separated and placed in /export/homes/USERNAME/pulse_sequences/cpc/includes. Each new file begins with a long comment section. The files acq.inc, CTUC_COSY.acq, CTUC_COSY.s, rSNOB_063108.inc, rSNOB_delay2.incSPC5.inc, SPINAL1.inc, SPINAL2.inc, STANDARD_PARAMS, std_errs.inc, t1.inc, t2.inc, TAN_CP.inc, and TWO_D.inc should be created from this file.

CO-CA Version

The hard pulse version of the experiment can be used if soft_pulse_flag=0 and ipap_flag=0.

Pulse sequence image

Aliphatic Version

The soft pulse version of the experiment can be used if soft_pulse_flag=1 and ipap_flag=0.

Pulse sequence image

CACO IPAP (IP) Version

The IP version of the experiment can be used if soft_pulse_flag=0 and ipap_flag=0, with the carrier placed on resonance with the Carbonyl frequency.

Pulse sequence image

CACO IPAP (AP) Version

The AP version of the experiment can be used if ipap_flag=1 (independent of soft_pulse_flag), with the carrier placed on resonance with the Carbonyl frequency.

Pulse sequence image


References

  1. Chen, L and Olson, RA and Elliot, DW and Boettcher, JM and Zhou, DH and Rienstra, CM and Mueller, LJ. Constant-Time Through-Bond ^{13}C Correlation Spectroscopy for Assigning Protein Resonances with Solid-State NMR Spectroscopy. J Am Chem Soc 128(31):9992--9993, 2006. BibTeX [bbCTUC]
  2. Chen, L and Kaiser, JM and Lai, J and Polenova, T and Yang, J and Rienstra, CM and Mueller, LJ. J-based 2D homonuclear and heteronuclear correlation in solid-state proteins. Magn Reson Chem 45 suppl.:s84-s92, 2007. BibTeX [ipapCTUC]

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