H(C)NH, (H)CNH, H(C)NH-TOCSY, C(C)NH-TOCSY Inova 500 MHz (G. Montelione, Rutgers Univ.)
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Name: pfg_C_hacanh_se1 | |||||
Synopsis: a family of triple resonance experiments for H-CC-N-HN correlations, straight-through, with sensitivity enhancement | |||||
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Copyright: G.T. Montelione, Protein NMR Laboratory - Center for Advanced Biotechnology and Medicine, Rutgers University | |||||
Console: Varian, Inc. Inova, 500 MHz | |||||
Console requirements: four RF channels (applies to some CABM sequences) | |||||
Source: CABM Pulse Sequence Library | |||||
Download (external links):readme pulse sequence parameters (tar)figure (pdf) |
References
- LYONS, BA and MONTELIONE, GT. An HCCNH triple-resonance experiment using carbon-13 isotropic mixing for correlating backbone amide and side-chain aliphatic resonances in isotopically enriched proteins. Journal of magnetic resonance Series B(Print) 101(2):206--209, 1993. BibTeX
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Lyons, BA and Tashiro, M and Cedergren, L and Bilsson, B and Montelione, GT. An improved strategy for determining resonance assignments for isotopically enriched proteins and its application to an engineered domain of staphylococcal protein A. Biochemistry 32(31):7839--7845, 1993. BibTeX
Categories: Unassigned experiment family | Sensitivity enhancement | Pulse sequences | CABM pulse sequences | Varian | Liquids | Inova | 500 MHz | 1H | 15N | 13C | Heteronuclear | 1H detected | 3D | Protein backbone | Protein sidechain | TOCSY | PFG