2D CC-CTUC Solids

From NMR Wiki

Revision as of 18:51, 5 September 2008 (edit) Trent Franks (Talk | contribs) m ← Previous diff		Current revision (16:20, 16 April 2009) (edit) (undo) Evgeny Fadeev (Talk | contribs)
(12 intermediate revisions not shown.)
Line 5:		Line 5:
	|code=I+CTUC_COSY.txt		|code=I+CTUC_COSY.txt
	|source=UIUC		|source=UIUC
-	|family=[[solids]]	+	|family=[[2D COSY]]
	|platform=Varian		|platform=Varian
	|console=Infinity Plus		|console=Infinity Plus
Line 17:		Line 17:
	Proton to Carbon CP followed by scalar coupling modulated homonuclear mixing with rSNOB soft pulses and independent [http://nmrwiki.org/wiki/index.php?title=SPINAL SPINAL] decoupling periods during mixing and acquisition.		Proton to Carbon CP followed by scalar coupling modulated homonuclear mixing with rSNOB soft pulses and independent [http://nmrwiki.org/wiki/index.php?title=SPINAL SPINAL] decoupling periods during mixing and acquisition.
-	All needed files were catenated together, and so must be separated. The file CTUC_COSY.acq is the first block of text after the author contributions. CTUC_COSY.s begins with	+	All needed files were catenated together, and so must be separated. The file CTUC_COSY.acq is the first block of text after the author contributions. CTUC_COSY.s begins with
-	name "2D_CC_shell";	+	:name "2D_CC_shell";
-		+	and ends with
-	and ends with	+	:return;
-	return;	+
	The next several files should be separated and placed in /export/homes/USERNAME/pulse_sequences/cpc/includes. Each new file begins with a long comment section.		The next several files should be separated and placed in /export/homes/USERNAME/pulse_sequences/cpc/includes. Each new file begins with a long comment section.
-	The files acq.inc, CTUC_COSY.acq, CTUC_COSY.s, rSNOB_063108.inc, rSNOB_delay2.incSPC5.inc, [http://nmrwiki.org/wiki/images/6/69/SPINAL1.inc.txt SPINAL1.inc], [http://nmrwiki.org/wiki/images/f/fc/SPINAL2.inc.txt SPINAL2.inc], STANDARD_PARAMS, std_errs.inc, t1.inc, t2.inc, TAN_CP.inc, and TWO_D.inc should be created from this file.	+	The files [[media:Acq.inc.txt|acq.inc]], CTUC_COSY.acq, CTUC_COSY.s, [[media:Rsnob.inc.txt|rSNOB_063108.inc]],[[media:Rsnob2.inc.txt|rSNOB_delay2.inc]], [[media:SPC5.inc.txt|SPC5.inc]], [[media:SPINAL1.inc.txt|SPINAL1.inc]], [[media:SPINAL2.inc.txt|SPINAL2.inc]], STANDARD_PARAMS, std_errs.inc, [[media:T1.inc.txt|t1.inc]], [[media:T2.inc.txt|t2.inc]], TAN_CP.inc, and TWO_D.inc should be created from this file.
	===CO-CA Version===		===CO-CA Version===
Line 30:		Line 29:
	The hard pulse version of the experiment can be used if soft_pulse_flag=0 and ipap_flag=0.		The hard pulse version of the experiment can be used if soft_pulse_flag=0 and ipap_flag=0.
-	<pulse>	+	<pulse title="image of CO-CA version of 2D CC-CTUC pulse sequence for solids">
-	anchors: @a,a1,a2 @b,b1 @c @c1,d,d1 @e @f @g @h------h1 @i	+	anchors: @a,a1,a2 @b,b1 @c @c1,d,d1 @e @f @g @h------h1 @i
-	disp: 4@a 5@b 7@c 7@d 1@e 7@f 7@g 4@h 10@i	+	disp: 4@a 5@b 7@c 7@d 1@e 7@f 7@g 4@h 10@i
-	time: rlx @a t1 @b t2 @c t3 @d t4 @e t5 @f t5 @g t6 @h d @i	+	time: rlx @a t1 @b t2 @c t3 @d t4 @e t5 @f t5 @g t6 @h d @i
-	rf H: 90@a=phy wp@a1,b=posramp wp@b1--------------------------------------g=fulldec wp@h1----i=acqdec	+	rf H: 90@a=phy wp@a1,b=posramp wp@b1--------------------------------------------------g=fulldec wp@h1----i=acqdec
	rf 13C: wp@a2,b=irr 180@c=phx 90@d=phy 90@e=phx 180@f=phy 90@g=phx 90@h=phmx acq@h1----i		rf 13C: wp@a2,b=irr 180@c=phx 90@d=phy 90@e=phx 180@f=phy 90@g=phx 90@h=phmx acq@h1----i
-	rf 15N: wp@h1----i	+	rf 15N: wp@h1----i
	delay t1: show_at=13C label=ct label_yoffset=-30		delay t1: show_at=13C label=ct label_yoffset=-30
	delay t2: show_at=13C label=\tau-\frac{t_1}{2}		delay t2: show_at=13C label=\tau-\frac{t_1}{2}
	delay t3: show_at=13C label=\tau+\frac{t_1}{2}		delay t3: show_at=13C label=\tau+\frac{t_1}{2}
-	delay t4: hide=true	+	delay t4: hide=true label=t4
	delay t5: show_at=13C label=\tau		delay t5: show_at=13C label=\tau
	delay t6: show_at=13C label=\tau_{zf}		delay t6: show_at=13C label=\tau_{zf}
Line 78:		Line 77:
	The soft pulse version of the experiment can be used if soft_pulse_flag=1 and ipap_flag=0.		The soft pulse version of the experiment can be used if soft_pulse_flag=1 and ipap_flag=0.
-	<pulse>	+	<pulse title="image of adiabatic version of 2D CC-CTUC pulse sequence for solids">
	anchors: @a,a1,a2 @b,b1 @c @c1,d,d1 @e @f @g @h,h1,h2,h3 @i,i1,i2,i3 @j		anchors: @a,a1,a2 @b,b1 @c @c1,d,d1 @e @f @g @h,h1,h2,h3 @i,i1,i2,i3 @j
	disp: 4@a 5@b 7@c 7@d 7@e 1@f 5@g 5@h 4@i 10@j		disp: 4@a 5@b 7@c 7@d 7@e 1@f 5@g 5@h 4@i 10@j
Line 118:		Line 117:
	The IP version of the experiment can be used if soft_pulse_flag=0 and ipap_flag=0, with the carrier placed on resonance with the Carbonyl frequency.		The IP version of the experiment can be used if soft_pulse_flag=0 and ipap_flag=0, with the carrier placed on resonance with the Carbonyl frequency.
-	<pulse>	+	<pulse title="image of CACO IPAP in-phase version of 2D CC-CTUC pulse sequence for solids">
	anchors: @a,a1,a2 @b,b1 @c @c1,d,d1 @e @f @g @h------h1 @i		anchors: @a,a1,a2 @b,b1 @c @c1,d,d1 @e @f @g @h------h1 @i
	disp: 4@a 5@b 7@c 7@d 1@e 7@f 7@g 4@h 10@i		disp: 4@a 5@b 7@c 7@d 1@e 7@f 7@g 4@h 10@i
Line 156:		Line 155:
	The AP version of the experiment can be used if ipap_flag=1 (independent of soft_pulse_flag), with the carrier placed on resonance with the Carbonyl frequency.		The AP version of the experiment can be used if ipap_flag=1 (independent of soft_pulse_flag), with the carrier placed on resonance with the Carbonyl frequency.
-	<pulse>	+	<pulse title="image of CACO IPAP anti-phase version of 2D CC-CTUC pulse sequence for solids">
	anchors: @a,a1,a2 @b,b1 @c @c1,d,d1 @e @f @g @h------h1 @i		anchors: @a,a1,a2 @b,b1 @c @c1,d,d1 @e @f @g @h------h1 @i
	disp: 4@a 5@b 7@c 7@d 1@e 7@f 7@g 4@h 10@i		disp: 4@a 5@b 7@c 7@d 1@e 7@f 7@g 4@h 10@i

---

Current revision

Name: hCC-2D-CTUC COSY
Experiment family/families: 2D COSY
Synopsis: Constant-time Through-bond Carbon 2D correlation experiments
Implementation author(s): Lindsay Sperling, Gemma Commellas, Trent Franks, Chad Rienstra
Copyright: not assigned
Console: Varian, Infinity Plus, 500 MHz
Download: code, no sample/parameter data yet

Implementation

Proton to Carbon CP followed by scalar coupling modulated homonuclear mixing with rSNOB soft pulses and independent SPINAL decoupling periods during mixing and acquisition.

All needed files were catenated together, and so must be separated. The file CTUC_COSY.acq is the first block of text after the author contributions. CTUC_COSY.s begins with

name "2D_CC_shell";

and ends with

return;

The next several files should be separated and placed in /export/homes/USERNAME/pulse_sequences/cpc/includes. Each new file begins with a long comment section. The files acq.inc, CTUC_COSY.acq, CTUC_COSY.s, rSNOB_063108.inc,rSNOB_delay2.inc, SPC5.inc, SPINAL1.inc, SPINAL2.inc, STANDARD_PARAMS, std_errs.inc, t1.inc, t2.inc, TAN_CP.inc, and TWO_D.inc should be created from this file.

CO-CA Version

The hard pulse version of the experiment can be used if soft_pulse_flag=0 and ipap_flag=0.

Aliphatic Version

The soft pulse version of the experiment can be used if soft_pulse_flag=1 and ipap_flag=0.

CACO IPAP (IP) Version

The IP version of the experiment can be used if soft_pulse_flag=0 and ipap_flag=0, with the carrier placed on resonance with the Carbonyl frequency.

CACO IPAP (AP) Version

The AP version of the experiment can be used if ipap_flag=1 (independent of soft_pulse_flag), with the carrier placed on resonance with the Carbonyl frequency.

References

1. Chen, L and Olson, RA and Elliot, DW and Boettcher, JM and Zhou, DH and Rienstra, CM and Mueller, LJ. Constant-Time Through-Bond ^{13}C Correlation Spectroscopy for Assigning Protein Resonances with Solid-State NMR Spectroscopy. J Am Chem Soc 128(31):9992--9993, 2006. BibTeX [bbCTUC]

2. Chen, L and Kaiser, JM and Lai, J and Polenova, T and Yang, J and Rienstra, CM and Mueller, LJ. J-based 2D homonuclear and heteronuclear correlation in solid-state proteins. Magn Reson Chem 45 suppl.:s84-s92, 2007. BibTeX [ipapCTUC]